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Why is it necessary to use only the Klenow fragment, rather tha... | Filo

Why is it necessary to use only the Klenow fragment, rather tha... | Filo

Difference between Klenow Fragment and DNA Polymerase Ⅰ

Difference between Klenow Fragment and DNA Polymerase Ⅰ

4.1K viewsJun 21, 2021

SOLVED: The Klenow fragment is produced when DNA polymerase 1 fromE.coli is enzymatically cleaved by the protease subtilisin. Usingdiagrams, explain what the Klenow fragment is and why the fragmentis better suited for site-directed mutagenesis than intact DNApolymerase 1?

SOLVED: The Klenow fragment is produced when DNA polymerase 1 fromE.coli is enzymatically cleaved by the protease subtilisin. Usingdiagrams, explain what the Klenow fragment is and why the fragmentis better suited for site-directed mutagenesis than intact DNApolymerase 1?

Why is it advantageous to use only the Klenow fragment, rather than intact E . coli Pol I, in DNA sequencing reactions (Section 3-4 C) ? | Numerade

Why is it advantageous to use only the Klenow fragment, rather than intact E . coli Pol I, in DNA sequencing reactions (Section 3-4 C) ? | Numerade

SOLVED:Why is it necessary to use only the Klenow fragment, rather than intact ℰ con' Pol I, in DNA sequencing rections ( Section 3 ·4 C) ?

SOLVED:Why is it necessary to use only the Klenow fragment, rather than intact ℰ con' Pol I, in DNA sequencing rections ( Section 3 ·4 C) ?

SOLVED:Why is it necessary to use only the Klenow fragment, rather than intact E . coli Pol I, in DNA sequencing reactions (Sections 3-4C and D)?

SOLVED:Why is it necessary to use only the Klenow fragment, rather than intact E . coli Pol I, in DNA sequencing reactions (Sections 3-4C and D)?

SOLVED:When cloning a foreign DNA fragment into a plasmid, it is often useful to insert the fragment at a site that interrupts a selectable marker (such as the tetracycline-resistance gene of pBR322). The loss of function of the interrupted gene can be used to identify clones containing recombinant plasmids with foreign DNA. With a bacteriophage λvector it is not necessary to do this, yet one can easily distinguish vectors that incorporate large foreign DNA fragments from those that do not. How

SOLVED:When cloning a foreign DNA fragment into a plasmid, it is often useful to insert the fragment at a site that interrupts a selectable marker (such as the tetracycline-resistance gene of pBR322). The loss of function of the interrupted gene can be used to identify clones containing recombinant plasmids with foreign DNA. With a bacteriophage λvector it is not necessary to do this, yet one can easily distinguish vectors that incorporate large foreign DNA fragments from those that do not. How

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