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Researchers at the Albert Einstein College of Medicine tracked beta-actin mRNA in real-time using a fluorescent tagging technique in a mouse model. They discovered that nerve stimulation quickly activates the beta-actin gene, with mRNA molecules appearing within minutes. These mRNA molecules move to the dendrites, where neurons regulate protein synthesis by packaging and unmasking the mRNA as needed. This process supports memory formation by strengthening specific synaptic connections. The origi

Researchers at the Albert Einstein College of Medicine tracked beta-actin mRNA in real-time using a fluorescent tagging technique in a mouse model. They discovered that nerve stimulation quickly activates the beta-actin gene, with mRNA molecules appearing within minutes. These mRNA molecules move to the dendrites, where neurons regulate protein synthesis by packaging and unmasking the mRNA as needed. This process supports memory formation by strengthening specific synaptic connections. The origi

720.6K views5 months ago

FacebookDr.Weird

SOLVED: You have been asked to clone a gene called "beta actin" into a pET15b plasmid, which is an expression vector. You have been provided with 10 ÂµL of the pure plasmid DNA in a microcentrifuge tube, as well as a 5 ÂµL sample of the purified beta actin DNA. Provided below is the gene sequence, the recognition sequences for the BamHI and XhoI restriction enzymes, and the vector map of the pET15b plasmid. Using this information, outline the steps in the cloning procedure from primer design to

SOLVED: You have been asked to clone a gene called "beta actin" into a pET15b plasmid, which is an expression vector. You have been provided with 10 ÂµL of the pure plasmid DNA in a microcentrifuge tube, as well as a 5 ÂµL sample of the purified beta actin DNA. Provided below is the gene sequence, the recognition sequences for the BamHI and XhoI restriction enzymes, and the vector map of the pET15b plasmid. Using this information, outline the steps in the cloning procedure from primer design to

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