Direct introduction of DNA into plant protoplasts facilitates a rapid analysis of transient gene expression, as well as the generation of stably transformed transgenic plants.

For many years of genetic manipulation in plants, direct uptake of naked DNA by plant protoplasts has been the sole alternative to Agrobacterium tumefaciens -mediated gene transfer.

Polyethylene glycol (PEG) can induce genetic transformation in both bacteria (Escherichia coli) and yeast (Saccharomyces cerevisiae) without cell wall removal. PEG-mediated transformation of E. coli is technically simple and yields transformants with an efficiency of 10(6)-10(7) transformants/microg …

Methods for direct gene transfer into protoplasts via polyethylene glycol (PEG) treatment and electroporation were developed in early 1980’s. Genetic transformation of protoplasts led to the production of the first transgenic cereals. This paper describes...

This chapter describes a method for PEG–mediated transformation of protoplasts derived from leaves, roots, and cell suspensions of A. thaliana. Leaf mesophyll protoplasts are able to regenerate after embedding into algmate, but their yield 1s relatively low.

The gene transfer techniques in plant genetic transformation are broadly grouped into two categories: I. Vector-mediated gene transfer. II. Direct or vector less DNA transfer. The salient features of the commonly used gene (DNA) transfer methods are given in Table 49.1.

The top four methods of gene transfer are: (1) DNA Transfer in Protoplasts (2) Free DNA Transfer to Intact Tissue (3) Agrobacterium Mediated Gene Transfer Method and (4) Integration and Expression.

Nov 1, 1983 · Polyethylene glycol (PEG) can induce genetic transformation in both bacteria (Escherichia coli) and yeast (Saccharomyces cerevisiae) without cell wall removal. PEG-mediated transformation of E. coli is technically simple and yields transformants with an efficiency of 10 6 –10 7 transformants/μg DNA.

Nov 1, 1983 · Polyethylene glycol (PEG) can induce genetic transformation in both bacteria (Escherichia coli) and yeast (Saccharomyces cerevisiae) without cell wall removal. PEG-mediated transformation of E. coli is technically simple and yields transformants with an efficiency of 10 6 –10 7 transformants/μg DNA.

a) Polyethylene glycol (PEG) mediated gene transfer. For the first time, this method was tested on Petunia and tobacco in presence of polyethylene glycol and poly L- ornithine. Steps involved in the PEG mediated transfer. Protoplasts are isolated. Plasmid DNA is mixed with protoplast suspension. To this mixture, 40% PEG is added.