5.0 μL of 10 × PCR buffer, 1.0 μL of dNTPs (10 mM), 1.0 μL of primer F27 (10 μM), 1.0 μL of primer R1522 (10 μM), 0.3 μL of DNA template, and 0.5 μL of Taq DNA Polymerase (5 U(μL) −1). The 1.4 kb PCR ...