In this manuscript the authors established a novel three-dimensional culture system for stratified epithelia that allows epithelial cells to undergo epithelial-to-mesenchymal transition (EMT) and ...

GAPDH immunoreactivity was detected using an anti-mouse IgG (1:6000, GE Healthcare) diluted in 5% nonfat dry milk. An enhanced chemiluminescence reagent (ECL Plus, Amersham) was used to visualize the ...

Recombinant mouse Fgl1 protein by tagging the histidine tag was purchased from ... Samples were normalized to glyceraldehyde-3-phosphate dehydrogenase (GAPDH) expression and showed in fold change ...

Moreover, in control and insulin-resistant differentiated mouse 3T3-L1 adipocytes ... The band intensity was measured by ImageJ software. The protein expression was normalized to α-tubulin or GAPDH.

Since receptor-interacting protein kinase-3 (RIP3) plays a pivotal role in necroptosis ... in damaged cartilage from patients with OA than in undamaged cartilage. In the mouse model, adenoviral RIP3 ...

The procedure for homogenising intestinal tissue and methods of assaying myeloperoxidase (MPO) activity and MMP9 protein levels have been reported ... USA) or rat anti-mouse Ly-6B.2 (1:400 dilution; ...

Fibroblast-like synoviocytes (FLS) from rats and patients with RA expressing or not Huntingtin-interacting protein 1 (HIP1 ... Dharmacon SMARTpool siRNA targeting either HIP1, GAPDH or a non-coding ...

Researchers from the Huntington's Disease Center, UCL Queen Square Institute of Neurology, and UK DRI at UCL have taken major ...

A study published in Science Advances reveals a novel strategy that allows tumors to evade the body's immune response ...

Now researchers from The Rockefeller University have unearthed intriguing genetic evidence: a protein variant found only in humans that may have helped shape the emergence of spoken language.