The experiments were performed with the QuantStudio™ 5 Real-Time PCR System (Thermofisher Scientific) and the qPCR conditions followed those recommended by the kit manufacturer. The standard curve was ...

2016). HF183/BacR287 multiplex IAC procedure was used to monitor for amplification inhibition. For each GFD instrument run, a melt curve analysis with a resolution of 0.3°C was used after thermal ...

Lambda DNA was used to generate the standard curve. All samples and standards were run in triplicates ... (Bio-Rad Laboratories), and 250 nM forward and reverse primers. Real-Time PCR amplification ...

Real-time PCR was performed with a LightCycler 480 (Life Technologies ... The identity and purity of the amplified product were assessed by analysing the melting curve, which was performed at the end ...

Real-time PCR (qPCR) is the gold-standard for sensitive, specific detection and quantification of nucleic acid targets through fluorescence-based techniques. TaqMan assays and SYBR Green dye are two ...

Their findings, published in Physical Review Letters, shed new light on the melting curve and structural phase of iron under extreme conditions. "The goal of this study was to explore the ...

Nvidia’s latest graphics cards sold out almost immediately, which means tons of people are still clamoring to get their hands on one. But with recent issues rearing their heads, you might want ...

We talk to three experts about what makes Thwaites so uniquely significant, why it’s melting and the creative techniques scientists are using to study it. As ice that is currently sitting on ...

If you recall a couple of years and several centuries ago when the RTX 4090 launched, a bunch of them melted. Okay, that’s hyperbolic, but a small but notable number of 40-series cards had ...