Before the second-round ALE, the cell mortality was firstly assessed with different concentrations (0, 5, 10, 15, 20, 25, and 30 μM) of quizalofop-p-ethyl in the agar plates containing seed medium.
TMV at a concentration of 0.01, 0.05, or 0.1 mg ml −1 was placed on Formvar carbon film coated TEM supports (VWR International) and stained with 2% (w/v) uranyl acetate (Agar Scientific). Images were ...
Serial dilution was performed for the isolation of bacterial strains, where the nutrient agar plates amended with 5 %, 10 %, 20 % and 30 % sodium chloride were spread with 100 µl of diluted samples.
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