SOLiD: Sequencing by Oligonucleotide Ligation and Detection.

Figure 2: Ligation of circularizable probes by Ampligase, a thermostable DNA ligase. To facilitate allele discrimination in genomic DNA, we designed a second oligonucleotide primer to bind ...

Ligation mixtures must be transformed into ... at least six Cm R /Km R bacterial clones for each individual target oligonucleotide when cloning individual shRNAs, or ensure at least threefold ...

USA), Life Technologies (Sequencing by Oligonucleotide Ligation and Detection [SOLiD™]; CA, USA) and Helicos BioSciences (HeliScope™ Single Molecule Sequencer; MA, USA). By sequencing DNA in a ...