Cells were then thoroughly washed with 0.1 mol/L PBS, without disrupting the cell pellet. Following decanting of the supernatant, 1% osmium acid (in PBS) was added and the mixture left standing for ...
3) Wash with 5 mL FACS buffer. Centrifuge the cells and decant the supernatant. 4) Resuspend the cells in FACS buffer and set aside 1 × 10 6 of isolated C57BL/6 J cells for DAPI and unstained ...
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