“As a result, one had really a snapshot in time.” There was no way to visualize what genes were active in a living organism. Chalfie thought that if GFP could be taken out of these marine organisms ...

Chalfie asked whether the cloned gene could be expressed in another organism, while Tsien saw long-term potential in making GFP glow brighter so it could be used as an all-purpose biological reporter.

In this system, green fluorescent protein (GFP) is used to tag all of a ... perform DNA sequencing in the wild-type and the mutant organisms to discover the specific mutation responsible for ...