With most types of DNA polymerase this single reaction step is all that could be carried out, but the thermostability of Taq polymerase means that the reaction mixture can be heated to temperatures in ...
The team focused on T7 DNA polymerase’s thioredoxin binding domain (TBD), which has a structure similar to that of most thermostable polymerases, including Taq. Thioredoxin, which is ubiquitous in ...