In this experiment you will use an artificial substrate, para-nitrophenylphosphate ... Para-nitrophenol is yellow at alkaline pH and its concentration can be measured at 405 nm. Verify the results ...

A continued increase in pH results in a sharp decrease in activity as the enzyme’s active site changes shape. It is now denatured. As the substrate concentration increases so does the rate of ...

Here we explore peptide inhibitors of Cdc20, a substrate-recognition subunit and activator of the E3 ubiquitin ligase the anaphase promoting complex/cyclosome (APC/C) that is essential in mitosis and ...