Oligonucleotide probes are short stretches of single-stranded DNA or RNA used to detect the presence of complementary nucleic acid sequences (target sequences) by hybridization. Oligonucleotide ...

(This nonspecific cutting of single-stranded DNA is unique to Cas12a and is not shared by the more well-known Cas9 protein.) Researchers use probes with a fluorophore and a quencher attached by single ...

Comparison of a single-stranded RNA and a double-stranded DNA with their corresponding nucleobases. (Image: Wikimedia Commons, CC SA 3.0) The most common type of base pairing is the Watson-Crick base ...

Linear oligonucleotide probes bound covalently on a glass ... will bind to each complementary sequence in the tandem single-stranded DNA, initiating sequential primer extension reactions (Fig.

Because of this difference, unmethylated cytosines in single-stranded DNA become uracil residues after exposure to sodium bisulfite, while 5mCs remain cytosines. Pretreatment with sodium bisulfite is ...