Appropriate controls in a qPCR assay ensure reliability and accuracy and help track any potential issues while providing a baseline for relative quantification. Some important controls include the ...
Although qRT-PCR studies provide valuable information about the relative expression of transcripts in a cell, they are difficult to perform with very small numbers of cells (for example ...
The ubiquity of quantitative PCR (qPCR) for interrogating gene expression in a variety of research and clinical contexts makes it an indispensable laboratory tool. Among the most critical variables ...