Polymerase chain reaction, or PCR, is a laboratory technique ... the temperature of the sample in automatic, programmed steps. Initially, the mixture is heated to denature, or separate, the ...

Polymerase chain reaction (PCR) is a fundamental molecular biology tool ... PCR involves the enzymatic amplification of nucleic acid templates, and can be divided into four major steps, listed below.

enables scientists to determine the amount of DNA present during each step of the PCR reaction. Real-time PCR can also be used to calculate the amount of specific kinds of genetic material other ...

PCR amplifies DNA in a three-step process: denaturation ... The coils rapidly heat a reaction block up to the 99°C denaturation temperature, the fan brings that down to the 68°C needed for ...