One strategy converts mammalian cells into ‘packaging cells’ by genetically modifying them to express the AAV genes rep and cap, which help copy and package viral genetic material. For gene ...

Figure 1: Strategy for packaging a large gene by the split AAV vectors through heterodimerization. Figure 3: Characterization of the split AAV vector heterodimerization by RT–PCR and DNA-PCR.

Using this method, scientists can directly compare the performance of different AAV simultaneously in a single animal ... which is required for viral genome replication and packaging; a cap gene, ...